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cOmplete™蛋白酶抑制剂鸡尾酒|cOmplete™ Protease Inhibitor Cocktail|上海现货 

上海创赛科技有限公司供应cOmplete™蛋白酶抑制剂鸡尾酒,cOmplete™ Protease Inhibitor Cocktail,标准物质,类似物,医药中间体,原料药,大包装,生化试剂,实验室耗材,实验室仪器等生化产品。

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4693116001-20片 20片 现货 3403.53 3403.53 0
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产品信息
Related      Categories Enzyme Inhibitors, Protease Inhibitors, 生化试剂, 蛋白酶抑制剂Cocktails, 酶、抑制剂和底物 Less...
form          tablet
packaging   pkg of 20 tablets (individually packed in foil blister packs)
mfr. no.      Roche
absorption   A/280 nm 0.08
shipped in   wet ice
price           3,403.53


Features and Benefits

Use cOmplete Protease Inhibitor Tablets to protect your proteins from a wide range of proteases. In just minutes, uninhibited proteolytic activity can degrade the protein you have spent days isolating. Each of these convenient water-soluble tablets contains a blend of protease inhibitors that inhibits proteolytic activity from most cell types, including animals, plants, yeast, and bacteria. No weighing or measuring is necessary, ensuring consistent results.

• Convenience: Simply push the quick-dissolving tablet through the foil packaging of the EASYpack into your buffer.
• Complete protection: Instantly protect your proteins against a broad range of proteases.
• Flexible: Protect proteins in extracts from almost any tissue or cell, including animals, plants, yeast, bacteria, or fungi.
• Safe: Choose non-toxic inhibitors that pose no risk to you or those around you.

• Suitable for IMAC purification: Purify poly-histidine tagged proteins with cOmplete His-Tag Purification Resin.


Application
cOmplete™ is used for the inhibition of serine, cysteine, and metalloproteases in bacterial, mammalian, yeast, and plant cell extracts.[3][1][2]
cOmplete contains both reversible and irreversible protease inhibitors. Therefore, we recommend the addition of cOmplete to all stock buffers and solutions normally protected with protease inhibitors, and not  just during the initial purification steps.
cOmplete contains EDTA (1 tablet yields a  1 mM EDTA solution  in 50 ml). Therefore, the extraction buffer should not contain divalent cations like Ca2+, Mg2+, or Mn2+, otherwise the inhibition of the metalloproteases might be incomplete. If the protein of interest will be purified by IMAC (Immobilized Metal Affinity Chromatography), for example, Poly-His-tagged recombinant proteins, EDTA has to be eliminated (e.g., by dialysis) prior to the chromatography. Alternatively, the product cOmplete, EDTA-free, can be used. These tablets are identical to cOmplete, with the exception that no EDTA or other chelating agent is  present.

cOmplete Tablets inhibit proteolytic activity in large extraction volumes (up to 50 ml). If you are working with smaller volumes (up to 10 ml), we recommend to use cOmplete, Mini. The composition of the cOmplete, Mini Tablet, is identical to the normal cOmplete Tablet.


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