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Magna ChIP™蛋白A + G磁珠|Magna ChIP™ Protein A+G Magnetic Beads|上海现货 

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16-663-50 reactions 50 reactions 现货 询价 0.00 0
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Product Information
Presentation Liquid suspension. Supplied as magnetic bead slurry in phosphate buffered saline, pH 7.4, containing 0.01% Tween®-20 and 0.09% sodium azide.
Applications
Application This blend of protein A+G magnetic beads allows for the use of a wider range of antibodies than A or G alone & provides a rapid, reproducible & efficient collection of immunocomplexes for chromatin immunoprecipitations (ChIP) and RNA immunoprecipitations (RIP) assays
Application Notes Use 20 µL of bead suspension per ChIP application. Includes sufficient reagents for 50 precipitation reactions. Disperse beads thoroughly before pipetting by rapid vortex.

Biological Information
Analytes Available
  • Protein A+G

Description
Catalogue Number 16-663
Trade Name
  • Magna ChIP
Description Magna ChIP™ Protein A+G Magnetic Beads
Overview Recombinant protein A plus recombinant protein G covalently bound to magnetic beads. This magnetic A+G blend allows for the use of a wider range of antibodies than A or G alone and provides a rapid, reproducible and efficient reagent for collecting immunocomplexes for chromatin immunoprecipitation (ChIP) assays as well as RNA immunoprecipitation (RIP) assays. Compared with conventional agarose beads, magnetic beads significantly reduce handling times and mechanical stress on target immunocomplexes.
Alternate Names
  • ChIP magnetic beads
  • ChIP magnetic A/G beads
  • ChIP magnetic A+G beads
Background Information Chromatin immunoprecipitation (ChIP) has been widely adapted for the study of gene-specific and genome-wide distribution of specific DNA and RNA-binding proteins or protein modifications. Similar to standard protein immunoprecipitation assays, ChIP involves isolation of immunocomplexes using a solid medium, such as agarose or magnetic beads, coupled to either IgG binding recombinant protein A or protein G. Experiments comparing protein A vs. protein G vs. protein A/G magnetic bead blends revealed that a mixture of protein A and G beads worked well with a wide variety of antibody isotypes. The use of Protein A/G bead blends eliminated the need to consider which beads or kit to use in order match a particular antibody/bead binding affinity combination. In addition to simplifying the procedure, comparing the use of either protein A or protein G alone, protein A/G magnetic bead blends improved signal-to-noise ratios without decreasing the recovery of input chromatin in ChIP assays.

Dimensions
Particle Size ~3 µm
Volume 1 mL
Product Usage Statements
Quality Assurance Routinely evaluated by Chromatin immunoprecipitation (ChIP) using HeLa nuclear extracts and the Magna ChIP™ A Kit (Cat. #17-610).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of shipment. Do Not Freeze.
Storage Temperature 2 to 8 °C (Do not freeze)
Packaging Information
Material Size 50 reactions


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