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39450-01-6|539480-100MG|蛋白酶K,Tritirachium相册|Proteinase K, Tritirachium album|上海现货 

上海创赛科技有限公司供应Proteinase K, Tritirachium album,标准物质,类似物,医药中间体,原料药,大包装,生化试剂,实验室耗材,实验室仪器等生化产品。

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539480-100MG-100mg 100mg 现货 询价 0.00 0
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References
References Watazu, Y., et al. 1993. J. Lab. Clin. Anal. 7, 81.
Lebherz, H.G., et al. 1986. Biochem. J. 233, 51.
Ebeling, W., et al. 1974. Eur. J. Biochem. 47, 91.
Product Information
CAS number 39450-01-6
Activity ≥30 mAnsonU/mg dry weight
Unit of Definition One mAnson unit is defined as the amount of enzyme that will liberate 1.0 µmol of Folin-positive amino acids (calculated as tyrosine) per min at 37°C, pH 7.5.
EC number 3.4.21.14
Form White lyophilized solid
Formulation Lyophilized from calcium acetate


Biological Information
Specific Activity ≥40 mAnsonU/mg protein
Description
Overview Serine protease that exhibits strong proteolytic activity on a wide variety of denatured and native proteins of high molecular weight. Because of its rapid proteolytic inactivation of endogenous nucleases, it can be used for isolation of mRNA and high molecular weight DNA. Preferentially cleaves bonds next to the carbonyl group of N-substituted hydrophobic, aliphatic, and aromatic amino acids. Inhibited by DFP, Hg2+, and PMSF. Not inactivated by metal chelators, sulfhydryl reagents, TLCK, or TPCK.
Catalogue Number 539480
Brand Family Calbiochem®
Physicochemical Information
Contaminants DNase: no detectable nicking activity with pBR322, incubation for 6 h at 37°C; RNase: no ribonuclease activity with MS2 RNA detected after incubation for 6 h at 37°C
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Irritant
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special Instructions Following reconstitution, store in the refrigerator (4°C). Stock solutions prepared in 50 mM Tris-HCl, 2 mM calcium acetate, pH 8.0 are stable for up to 1 year at 4°C.


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