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ESGRO®白血病抑制因子(LIF),1000万单位/ 1 mL|ESGRO® Leukemia Inhibitory Factor (LIF), 10 million units/1 mL|上海现货 

上海创赛科技有限公司供应ESGRO®白血病抑制因子(LIF),1000万单位/ 1 mL,标准物质,类似物,医药中间体,原料药,大包装,生化试剂,实验室耗材,实验室仪器等生化产品。

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Product Information
Format Purified
Presentation ESGRO® Supplement is supplied in liquid form as 107 Units in 1.0 mL of phosphate buffered saline with 1% w/v bovine serum albumin BSA as a carrier for stability.
Applications
Application The ESGRO Leukemia Inhibitory Factor (LIF) supplement is an optimized formulation of recombinant mouse LIF (mLIF) for in vitro maintenance of mouse ES and iPS cells. Each vial contains 10^7 units.
Key Applications
  • Stem Cell Culture
Application Notes Applications for ESGRO® Supplement include use as a reagent for the in vitro maintenance of the pluripotential phenotype of murine ES cells. 

SUGGESTED PROTOCOLS: 

ES Cells: In D3 and MBL-1 pluripotential ES cell cultures it is routinely found that 1000 U ESGRO® Supplement per 1.0 mL of tissue culture media is required to maintain ES cells with a stem cell phenotype. Similar concentrations of mLIF have also been used for germline transmission of genetically altered ES cells(E3). 

At the recommended concentration 107 units of ESGRO® is sufficient for 10.0 L of tissue culture media and 106 units of ESGRO® Supplement is sufficient for 1.0 L of tissue culture media.
Key Applications Purity
Cult The active component mLIF has been shown to be >95% pure by SDS-PAGE. ESGRO® Supplement is supplied 0.22 micron sterile filtered, and tested negative in mycoplasma tests.
Description
Catalogue Number ESG1107
Brand Family Chemicon®
Trade Name
  • Chemicon
Description ESGRO® Leukemia Inhibitory Factor (LIF), 10 million units/1 mL
Overview The ESGRO mouse LIF medium supplement is supplied in active units/mL for consistent performance from lot to lot. Each lot of ESGRO supplement undergoes stringent quality control testing to ensure reliable inhibition of mouse pluripotent stem cell differentiation. ESGRO mouse LIF supplement may be used for feeder based and feeder free culture of mouse embryonic stem (ES) and induced pluripotent stem (iPS) cells.
Alternate Names
  • Murine LIF
  • Mouse LIF
  • mLIF
Background Information Mouse Leukemia Inhibitory Factor (LIF, mLIF) promotes self-renewal and long-term maintenance of pluripotent mouse embryonic stem (ES) and induced pluripotent stem (iPS) cells by suppressing spontaneous differentiation.
Biological Information
Concentration 10 million units/1 mL
Purity The active component mLIF has been shown to be >95% pure by SDS-PAGE. ESGRO® Supplement is supplied 0.22 micron sterile filtered, and tested negative in mycoplasma tests.
Specific Activity ESGRO® Supplement is assessed both on mouse embryonic stem cells (D3 & MBL-1) and on murine M1 myeloid leukemic cells(4). A standard of 50 Units is defined as the concentration of ESGRO® Supplement, in 1.0mL of tissue culture medium, that induces the differentiation of 50% of M1 colonies(4). 10E7 units is equivalent to approximately 100 μg of pure protein, and is sufficient to treat 10L of ES cell culture media. <br /><br />Embryonic Stem Cell Assay: Differentiation inhibition at 1000 units/mL Murine myeloid leukemic, M1 Assay: Specific Activity >/= 10<sup>8</sup> units/mg
Entrez Gene Number
  • NM_008501
Entrez Gene Summary Leukaemia inhibitory factor is a cytokine that induces macrophage differentiation. Neurotransmitters and neuropeptides, well known for their role in the communication between neurons, are also capable of activating monocytes and macrophages and inducing chemotaxis in immune cells. LIF signals through different receptors and transcription factors. LIF in conjunction with BMP2 acts in synergy on primary fetal neural progenitor cells to induce astrocytes.
Gene Symbol
  • LIF
  • Emfilermin
  • CDF
  • MLPLI
  • HILDA
  • D-FACTOR
UniProt Number
  • P09056
UniProt Summary FUNCTION: SwissProt: P15018 # LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
SIZE: 202 amino acids; 22008 Da 
SUBCELLULAR LOCATION: Secreted.
SIMILARITY: SwissProt: P15018 ## Belongs to the LIF/OSM family.
Media Form Liquid
Stem Cell Type
  • Mouse Embryonic Stem Cells
  • Induced Pluripotent Stem Cells
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
  • LABEL LICENSE - USE RESTRICTED TO RESEARCH USE ONLY

    Leukemia Inhibitory Factor ("LIF") is protected by US Patents No. 5,443,825 and 5,750,654 and Canada Patent No. 1,341,581 and 1,341,469 owned by AMRAD Corporation Ltd. and licensed exclusively to Millipore for research uses. The use of this product is limited for research uses only and not for any commercial purposes, such as making or developing other research products from this product or use of this product as a component in a research kit. This product is not to be used in humans or for any diagnostic purposes.

    The purchaser may refuse to accept the conditions of this label license by returning this product unopened and will receive a full refund of the purchase price paid.

    For-profit entities or commercial research entities purchasing this product shall be required to execute a separate commercial license with Millipore within three months of purchase. Researchers may not transfer this product or its derivatives to researchers performing commercial research at other Nonprofit Organizations, or to For-Profit Organizations, without the express written consent of Millipore, and without those entities having an express license from Millipore for the use of the product. Other than as specifically provided herein, a transferee of this product shall have no right to transfer this product, or its derivatives to any other person or entity.
Storage and Shipping Information
Storage Conditions ESGRO® Supplement is shipped at ambient temperature. A cold pack may be included for customer relations, however it is truly not needed. Extensive stability tests have been performed with ESGRO® Supplement during development. This product is stable for at least 18 months from the date of manufacture, in the concentrated form or diluted in sterile tissue culture media, with no loss of activity on ES cells. For long term storage it is recommended that ESGRO® Supplement be stored at 4°C. Freeze-thawing will reduce potency. It is recommended that prior to use, ESGRO® Supplement should be diluted in sterile tissue culture media and aliquoted to a convenient concentration, then stored at 4°C. Freeze-thawing should be avoided. Product is stable for a minimum of 7 days at 37°C, 5% CO2 incubator during the culture of ES cells.
Packaging Information
Material Size 10⁷ units
Material Package 1 mL

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